Biochemistry Questions Biochemistry Questions / Discuss the role of hydride ions in oxidoreductases of fatty acid biosynthesis, comparing the use of borohydride and cofactors like NADH and NADPH. How do these reducing agents differ in terms of effectiveness and biological applicability?

Hydride ions, H-, are necessary to oxidise or reduce compounds. BH4- is a very good reducing agent, reducing almost any compound it comes into contact with – but is far too strong for biological applications (NADH/NADPH is normally used). NAD(P)H is able to be targeted to the specific site by cells, and is involved in many metabolic reactions.

NAD(P)H is a less strong reductant when compared to BH4-, but this is better in biological settings as it could cause severe damage to the cell.