Biochemistry Questions Biochemistry Questions / Describe the process of RNA self-cleavage in alkaline conditions and why RNA is more susceptible to breakage in basic environments. Discuss the formation of pentaphosphate intermediates during this process.

RNA self-cleavage is possible in alkaline conditions, with the 2’ OH group being the source of the attacking nucleophile. This cleaves the phosphodiester bond, taking up a H+ from the water.

A pentaphosphate intermediate is formed, allowing the bond from the phosphate group to the nucleobase to be broken. This is because phosphate cannot support 5 bonds.