Biochemistry Questions Biochemistry Questions / Discuss the process of translational control and the role of eIF4E, eIF4G, and PABP in translation initiation.

Translational control is an important mechanism to ensure mRNA transcripts are accurately translated into functional protein products.

eIF4A, eIF4E, and eIF4G form the cap-binding complex at the 5’ terminal of the mRNA. eIF4A removes structures from the mRNA, with a helicase activity. eIF4E recruits ribosomes, initiating translation. eIF4G interacts with the poly(A) binding protein, linking the 5’ cap structure to the 3’ UTR. This forms a sort-of circular mRNA molecule, enabling ribosomes to end translation near the start site (leading to an increase in transcription).

PABP, poly(A) binding protein, binds to the 3’ UTR multiple times, providing a binding site for eIF4G. By having a circular molecule, ribosomes end translation near the start site, increasing protein yield. Breaking this circular structure, such as with 4EBP, can be used to downregulate transcription.